I am putting a list of my routine protocols. You are welcome to use and adapt them if you wish. I publish them here for two reasons. Firstly, to have a central place that I can go to find my protocols. Secondly, if it can help others, it is great too.
From RNA extraction to qPCR:
1- RNA extraction from cell culture.
2- cDNA from RNA
3- Testing primers for qPCR