I am putting a list of my routine protocols. You are welcome to use and adapt them if you wish. I publish them here for two reasons. Firstly, to have a central place where I can go to find my protocols. Secondly, if it can help others, it is great too.
- From RNA extraction, cDNA conversion to rt-qPCR
- Protein quantification and BSA assay
- Lentiviral production
- Primary cell culture from P0-P1 mouse hipocampal brain